Columbia Basin Fish and Wildlife Program Columbia Basin Fish and Wildlife Program
SOW Report
Contract 56109: 2002-030-00 EXP 24747 PROGENY MARKER FOR SALMONIDS
Project Number:
Title:
Salmonid Progeny Markers
Stage:
Closed
Area:
Province Subbasin %
Columbia Plateau Umatilla 100.00%
Contract Number:
56109
Contract Title:
2002-030-00 EXP 24747 PROGENY MARKER FOR SALMONIDS
Contract Continuation:
Previous: Next:
53059: 2002-030-00 EXP PROGENY MARKER FOR SALMONIDS
  • 60143: 2002-030-00 EXP 24747 PROGENY MARKER FOR SALMONIDS
Contract Status:
Closed
Contract Description:
In order to evaluate the survival of salmon produced artificially, it is necessary to mark the fry of hatchery spawning events (Schroder et al. 1994).  The consequences for marking millions of fry rapidly are labor intensive and sometimes impractical due to their size.  However, the authors provide a technique for marking millions of fish without individual handling by delivering a constant flow containing strontium to their rearing water.  Results from this study show an elevation in strontium by injecting mothers prior to egg fertilization.  Although the differences between treated and untreated fish are not as discrete as the studies using immersion baths (Shippentower, 2011), the injection technique provides an opportunity for marking millions of eggs without individual handling.

In this study, we will evaluate if a strontium solution could artificially elevate the Sr: Ca ratios in the otoliths of O. mykiss progeny by using an intra peritoneal cavity injection of strontium chloride into pre-spawning adult female steelhead.  In addition to investigating whether Sr: Ca ratios become elevated with treatment, we will also assess population genetic structure of progeny from previous generations of juvenile steelhead and measure Sr: Ca ratios in otoliths to establish background information on the variation present.  IP injections containing strontium coupled with a pedigree analysis could be used to distinguish between the progeny of hatchery versus wild females that spawn naturally.  For example, in an integrated hatchery program where wild fish are subject for hatchery brood and hatchery fish are not, but are allowed to spawn in the wild.  If a female hatchery origin fish received a trans-generational mark and was fin clipped and released to spawn naturally, her offspring could than be distinguished from the offspring of wild females by analyzing their otolith microchemistry and pedigree.

Ho: There is no difference in the relative reproductive success of hatchery versus wild female steelhead that spawns naturally in Iskúulktpe Creek.

Ho: There is no difference in proportion of hatchery females (fin clipped) relative to wild females (no fin clip) returning to Three-mile Falls Dam located on the Umatilla River and Iskúulktpe Creek.

Ho: There is no difference in Sr: Ca ratios collected from progeny of strontium treated and untreated females.

Ho: There is no relationship between water and otolith chemistries (Sr: Ca ratios) collected from Onchorhynchus mykiss parr and their natal stream.

Ho: A distinct genetic mark will identify progeny of females within and among each treatment group.

Ho: There is no difference in spawning distribution between hatchery and wild females.

Ho: There is no difference in the relative abundance of Onchorhynchus mykiss parr from strontium treated and untreated females in like physical habitat units.

Ho: There is no difference in the relative abundance of progeny from sympatric anadromous and non-anadromous Onchorhynchus mykiss in like physical habitat units.
Account Type(s):
Expense
Contract Start Date:
01/01/2012
Contract End Date:
12/31/2012
Current Contract Value:
$334,050
Expenditures:
$334,050

* Expenditures data includes accruals and are based on data through 31-Mar-2024.

Env. Compliance Lead:
Work Order Task(s):
Contract Type:
Contract (IGC)
Pricing Method:
Cost Reimbursement (CNF)
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Full Name Organization Write Permission Contact Role Email Work Phone
Jan Brady Bonneville Power Administration Yes COR jebrady@bpa.gov (503) 230-4514
Julie Burke Umatilla Confederated Tribes (CTUIR) Yes Administrative Contact julieburke@ctuir.org (541) 429-7292
Israel Duran Bonneville Power Administration No Env. Compliance Lead induran@bpa.gov (503) 230-3967
Brenda Heister Bonneville Power Administration Yes Contracting Officer bsheister@bpa.gov (503) 230-3531
Gary James Umatilla Confederated Tribes (CTUIR) Yes Supervisor garyjames@ctuir.org (541) 429-7285
Paul Krueger Bonneville Power Administration Yes F&W Approver pqkrueger@bpa.gov (503) 230-5723
Peter Lofy Bonneville Power Administration Yes Interested Party ptlofy@bpa.gov (503) 230-4193
Travis Olsen Umatilla Confederated Tribes (CTUIR) Yes Contract Manager travisolsen@ctuir.org (541) 429-7542
Paul Rabb Umatilla Confederated Tribes (CTUIR) No Administrative Contact paulrabb@ctuir.org (541) 429-7165
Barbara Shields Bonneville Power Administration Yes Interested Party bashields@bpa.gov (503) 230-4748
Gene Shippentower Umatilla Confederated Tribes (CTUIR) Yes Supervisor geneshippentower@ctuir.org (541) 429-7287


Viewing of Work Statement Elements

Deliverable Title WSE Sort Letter, Number, Title Start End Complete
Deflection Board Weir Installed B: 70. Site preparation and assembly of deflection board weir in Iskúulktpe Creek tributary 10/31/2012 10/31/2012
Sr: Ca ratio variability in Iskúulktpe Creek - fall 2011 otolith collection C: 157. Otolith Sampling from Iskúulktpe Creek O.mykiss 12/31/2012 12/28/2012
Radio tag summer steelhead captured in Iskúulktpe weir D: 157. Collect radio tag data on adult steelhead caputured at Iskúulktpe Weir 06/30/2012 05/15/2012
Microchemistry of water E: 157. Collect water samples for Sr:Ca variability 12/31/2012 12/28/2012
Population genetics F: 157. Genetic Identification for Iskúulktpe Creek steelhead 12/31/2012 12/28/2012
Experimental units for testing a trans-generational mark G: 158. Sampling Iskúulktpe Summer Steelhead 06/30/2012 05/15/2012
Funding Package - Submit draft to COTR H: 119. Manage and administer project 09/30/2012 09/30/2012
Genetic analysis I: 162. Pedigree report- Determine hatchery contribution to natural production 12/08/2012 12/28/2012
Microchemistry analysis J: 162. Use microchemistry to describe contribution of hatchery fish to Nat Production 12/31/2012
spatial distribution K: 162. Spatial distribution of summer steelhead in Iskúulktpe Creek 08/01/2012 08/01/2012
Inter and intra- agency coordination L: 189. Coordinate field and laboratory activities 12/31/2012 12/28/2012
Environmental Compliance complete M: 165. Environmental Compliance complete on time 12/30/2012 12/28/2012
Attach Progress Report in Pisces N: 132. Submit Progress Report for the period Nov 2010 to Dec 2011 11/15/2012

Viewing of Implementation Metrics
Viewing of Environmental Metrics Customize

Primary Focal Species Work Statement Elements
Steelhead (O. mykiss) - Middle Columbia River DPS (Threatened)
  • 1 instance of WE 70 Install Fish Monitoring Equipment
  • 4 instances of WE 157 Collect/Generate/Validate Field and Lab Data
  • 1 instance of WE 158 Mark/Tag Animals
  • 3 instances of WE 162 Analyze/Interpret Data

Sort WE ID WE Title NEPA NOAA USFWS NHPA Has Provisions Inadvertent Discovery Completed
A 185 Periodic Status Reports for BPA 01/01/2012
B 70 Site preparation and assembly of deflection board weir in Iskúulktpe Creek tributary 08/29/2012
C 157 Otolith Sampling from Iskúulktpe Creek O.mykiss 08/29/2012
D 157 Collect radio tag data on adult steelhead caputured at Iskúulktpe Weir 01/01/2012
E 157 Collect water samples for Sr:Ca variability 08/29/2012
F 157 Genetic Identification for Iskúulktpe Creek steelhead 08/29/2012
G 158 Sampling Iskúulktpe Summer Steelhead 08/29/2012
H 119 Manage and administer project 01/01/2012
I 162 Pedigree report- Determine hatchery contribution to natural production 01/01/2012
J 162 Use microchemistry to describe contribution of hatchery fish to Nat Production 01/01/2012
K 162 Spatial distribution of summer steelhead in Iskúulktpe Creek 01/01/2012
L 189 Coordinate field and laboratory activities 01/01/2012
M 165 Environmental Compliance complete on time 01/01/2012
N 132 Submit Progress Report for the period Nov 2010 to Dec 2011 01/01/2012