Columbia Basin Fish and Wildlife Program Columbia Basin Fish and Wildlife Program
SOW Report
Contract 61126: 2008-907-00 EXP GENETIC ASSESSMENT OF COLUMBIA RIVER STOCKS
Project Number:
Title:
Genetic Assessment of Columbia River Stocks
BPA PM:
Stage:
Implementation
Area:
Province Subbasin %
Basinwide - 50.00%
Mainstem - 50.00%
Contract Number:
61126
Contract Title:
2008-907-00 EXP GENETIC ASSESSMENT OF COLUMBIA RIVER STOCKS
Contract Continuation:
Previous: Next:
56518: 2008-907-00 EXP GENETIC ASSESSMENT OF COLUMBIA RIVER STOCKS
  • 64699: 2008-907-00 GENETIC ASSESSMENT OF COLUMBIA RIVER STOCKS
Contract Status:
Closed
Contract Description:
This project combines four inter-related projects from the Accords that address Single Nucleotide Polymorphism (SNP) Discovery, Genetic Baseline Expansion, Genetic Stock Identification (GSI) to Evaluate Catch, and GSI of fishes passing Bonneville Dam.  These four projects are highly related since SNP markers are needed to complete species specific baselines, and these baselines are requisite to complete GSI.  

The specific objectives are:

1) discover and evaluate SNP markers in salmonids and other fishes

2) expand and create genetic baselines for multiple species (Chinook, steelhead, sockeye, and coho)

3) implement GSI programs for mainstem fisheries

4) GSI of fish passing Bonneville Dam

These four objectives address needs for distinguishing specific stocks, determining genetic diversity, stock specific run timing, and estimating stock composition to provide information for fisheries management and harvest.  Newly discovered SNP markers may also be useful for other applications such as pedigree studies for estimating reproductive success, and evaluating adaptive divergence of populations to specific environments.
Account Type(s):
Expense
Contract Start Date:
04/01/2013
Contract End Date:
03/31/2014
Current Contract Value:
$750,010
Expenditures:
$750,010

* Expenditures data includes accruals and are based on data through 31-Mar-2021.

Env. Compliance Lead:
Work Order Task(s):
Contract Type:
Contract (IGC)
Pricing Method:
Cost Reimbursement (CNF)
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Viewing of Work Statement Elements

Deliverable Title WSE Sort Letter, Number, Title Start End Complete
Fulfill all administrative tasks with quality products and in a timely manner. B: 119. Obj. 1-4; Manage/Admin. Projects 03/31/2014 03/24/2014
Obtain Permit C: 165. Obj. 1-4; Environmental Compl. 04/01/2013 04/01/2013
Submit manuscript D: 183. Obj. 1-4; Journal articles 03/31/2014 03/24/2014
Description of approach E: 156. Obj. 1) SNP Ascertainment Strategy 01/31/2014 01/31/2014
Sequencing and Assay development F: 157. Obj. 1) Sequencing and Assay Development 01/31/2014 01/31/2014
Estimate allele frequencies G: 162. Obj. 1) Analysis of SNP Frequencies 02/28/2014 02/28/2014
Report SNP sites H: 161. Obj. 1) SNP Sites and Assays 03/31/2014 03/24/2014
List of SNPs and populations I: 156. Obj. 2) Determine SNP Markers and Baseline Samples 01/15/2014 01/15/2014
Multi-locus SNP genotypes J: 157. Obj. 2) SNP Genotyping 01/15/2014 01/15/2014
Submit to Database K: 159. Obj. 2) Baseline Genotypes to Database 03/31/2014 03/24/2014
Analyze Genetic Data L: 162. Obj. 2) Population Diversity and Differentiation 03/14/2014 03/14/2014
Report/Present Genetic Data M: 161. Obj. 2) Genotypes and Allele Frequencies 03/31/2014 03/24/2014
Hold Meetings N: 189. Obj. 3) Coordination Among Agencies 03/31/2014 03/24/2014
Sample Plan O: 156. Obj. 3) Develop Sampling Plan 12/06/2013 12/06/2013
Inventory of Samples P: 157. Obj. 3) Collect Tissue Samples from Fisheries 01/15/2014 01/15/2014
Summary of Genotypes Q: 157. Obj. 3) Genotype Tissue Samples 03/14/2014 03/14/2014
Stock Composition R: 162. Obj. 3) Estimate Stock Composition 03/31/2014 03/24/2014
Report Stock Composition S: 161. Obj. 3) Distribute GSI Estimates 03/31/2014 03/24/2014
Sampling plan T: 156. Obj. 4) Sampling period, size, and logistics 10/31/2013 10/31/2013
Inventory of Samples U: 157. Obj. 4) Non-lethal sampling at Bonneville Dam 01/15/2014 01/15/2014
Summary of Genotypes V: 157. Obj. 4) Genotype tissue samples 03/31/2014 03/24/2014
Stock Composition W: 162. Obj. 4) GSI estimates 03/31/2014 03/24/2014
Post Results Online X: 161. Obj. 4) Post updates to CRITFC website 03/31/2014 03/24/2014
Annual RM&E Report for April-December 2012 Y: 132. Obj. 1-4) Submit Annual Report for Apr-12 to Dec-12 03/31/2014 03/24/2014
BiOp RM&E report for CY 2013 AA: 141. Obj. 1-4) BiOp RPA Report for CY 2013 03/15/2014 03/15/2014

Viewing of Implementation Metrics
Viewing of Environmental Metrics Customize

Primary Focal Species Work Statement Elements
Chinook (Oncorhynchus tshawytscha) - All Populations
  • 1 instance of WE 183 Produce Journal Article
  • 6 instances of WE 157 Collect/Generate/Validate Field and Lab Data
  • 1 instance of WE 159 Transfer/Consolidate Regionally Standardized Data
  • 4 instances of WE 161 Disseminate Raw/Summary Data and Results
  • 4 instances of WE 162 Analyze/Interpret Data
Sockeye (Oncorhynchus nerka) - All Populations
  • 1 instance of WE 183 Produce Journal Article
Steelhead (Oncorhynchus mykiss) - All Populations
  • 1 instance of WE 183 Produce Journal Article
  • 4 instances of WE 157 Collect/Generate/Validate Field and Lab Data
  • 1 instance of WE 159 Transfer/Consolidate Regionally Standardized Data
  • 3 instances of WE 161 Disseminate Raw/Summary Data and Results
  • 3 instances of WE 162 Analyze/Interpret Data

Sort WE ID WE Title NEPA NOAA USFWS NHPA Has Provisions Inadvertent Discovery Completed
A 185 Periodic Status Reports for BPA 04/01/2013
B 119 Obj. 1-4; Manage/Admin. Projects 04/01/2013
C 165 Obj. 1-4; Environmental Compl. 04/01/2013
D 183 Obj. 1-4; Journal articles 04/01/2013
E 156 Obj. 1) SNP Ascertainment Strategy 04/01/2013
F 157 Obj. 1) Sequencing and Assay Development 04/01/2013
G 162 Obj. 1) Analysis of SNP Frequencies 04/01/2013
H 161 Obj. 1) SNP Sites and Assays 04/01/2013
I 156 Obj. 2) Determine SNP Markers and Baseline Samples 04/01/2013
J 157 Obj. 2) SNP Genotyping 04/01/2013
K 159 Obj. 2) Baseline Genotypes to Database 04/01/2013
L 162 Obj. 2) Population Diversity and Differentiation 04/01/2013
M 161 Obj. 2) Genotypes and Allele Frequencies 04/01/2013
N 189 Obj. 3) Coordination Among Agencies 04/01/2013
O 156 Obj. 3) Develop Sampling Plan 04/01/2013
P 157 Obj. 3) Collect Tissue Samples from Fisheries 04/01/2013
Q 157 Obj. 3) Genotype Tissue Samples 04/01/2013
R 162 Obj. 3) Estimate Stock Composition 04/01/2013
S 161 Obj. 3) Distribute GSI Estimates 04/01/2013
T 156 Obj. 4) Sampling period, size, and logistics 04/01/2013
U 157 Obj. 4) Non-lethal sampling at Bonneville Dam 04/01/2013
V 157 Obj. 4) Genotype tissue samples 04/01/2013
W 162 Obj. 4) GSI estimates 04/01/2013
X 161 Obj. 4) Post updates to CRITFC website 04/01/2013
Y 132 Obj. 1-4) Submit Annual Report for Apr-12 to Dec-12 04/01/2013
Z 132 Obj. 1-4) Submit Draft Annual Technical Report for CY 2013 04/01/2013
AA 141 Obj. 1-4) BiOp RPA Report for CY 2013 04/01/2013