Columbia Basin Fish and Wildlife Program Columbia Basin Fish and Wildlife Program
SOW Report
Contract 69663: 2010-033-00 EXP STUDY REPRODUCTIVE SUCCESS
Project Number:
Title:
Study Reproductive Success of Hatchery and Natural Origin Steelhead in the Methow
Stage:
Implementation
Area:
Province Subbasin %
Columbia Cascade Methow 100.00%
Contract Number:
69663
Contract Title:
2010-033-00 EXP STUDY REPRODUCTIVE SUCCESS
Contract Continuation:
Previous: Next:
65859: 2010-033-00 EXP STUDY REPRODUCTIVE SUCCESS
  • 73235: 2010-033-00 EXP WDFW TWISP STUDY REPRODUCTIVE SUCCESS
Contract Status:
Closed
Contract Description:
During the August-2015 to July 2016 contract cycle, we will trap, sample, and collect the necessary tissue samples from the 2016 brood of returning adult steelhead at the Twisp River weir, and will collect and sample juvenile progeny in the Twisp River.  All sampling data will be entered in local databases, and the genetic material collected will be transferred to the WDFW Molecular Genetics Lab in Olympia WA, for archiving.

In 2014, we released 64 wild adult steelhead and 60 hatchery adult steelhead upstream of the Twisp River weir.  Wild adult steelhead returning to the Twisp weir were generally larger, had a higher fat content, arrived at the Twisp weir earlier, and spawned earlier than hatchery steelhead.  However, hatchery females returning to the weir had greater fecundity than wild females.  Wild and hatchery steelhead were similar in terms of migration speed, but wild female steelhead spawned farther upstream than hatchery female steelhead in 2014.  Age-1 parr that were sampled in 2012 and 2013 (i.e., offspring of the 2011 and 2012 broods, respectively) were genotyped during the 2014-2015 contract period, but analysis of those samples is pending.  Preliminary genotyping results from the age-1 progeny that have been genotyped previously suggest that hatchery and wild mothers produced similar numbers of age-1 offspring; however, wild males appeared to produce more offspring than hatchery males.  

Background on project:

We propose to quantitatively evaluate the relative reproductive success of naturally spawning hatchery and natural origin steelhead in the Twisp River, a tributary to the Methow River in the upper Columbia River Basin.  Hatcheries are one of the main tools that have been used to mitigate for salmon losses caused by the construction and operation of the Columbia River hydropower system.  In addition to harvest augmentation, hatcheries have recently been used in attempts to protect stocks from extinction (e.g., captive breeding) and attempts to enhance natural production (supplementation).  Surprisingly, little is known about how much the investment in hatcheries benefits or harms natural production.  We propose to take advantage of recent technological advances in genetics to empirically monitor the reproductive success of hatchery and natural steelhead using a DNA-based pedigree approach.  For this contract period, we will continue to trap and collect DNA and biological data from all steelhead released upstream of the weir, conduct intensive spawning ground surveys and collect DNA samples from progeny (i.e., parr and smolts) of previous brood years.  Specifically, we will (1) directly measure the relative reproductive success of hatchery- and natural-origin steelhead  in a the natural environment, (2) determine the degree to which any differences in reproductive success between hatchery and natural steelhead can be explained by measurable biological characteristics such as run timing, morphology, or behavior, and (3) estimate the relative fitness of hatchery-lineage steelhead after they have experienced an entire generation in the natural environment.

Genotyping of adult steelhead is funding by Douglas County PUD and is not part of this contract.  In addition, juvenile genotyping for the previous contract was deferred until this current contract period per agreements between WDFW and BPA. Hence, parental assignments could not be conducted until the 2014-2015 contract was implemented.

In the previous contract (# 65859), genotyping of Snake River Fall Chinook samples were added to this project.  This contract will continue that work.  See work element 'J'.

Tissue samples were collected from Chinook salmon broodstock used at the Lyons Ferry Hatchery.  Samples were preserved in 100% ethanol solution and sent to the Washington Department of Fish and Wildlife Molecular Genetics Laboratory in Olympia, WA for analysis.  

The three labs (WDFW, IDFG and CRITFC) will need to all submit their data into the same data base (e.g., CRITFC’s and IDFG database) and submit information annually (number of analyses, etc…) to the run reconstruction team associated with project 2012-013-00 who will be responsible for rolling this information up and reporting  it in their annual project reports.

1,216 samples will be analyzed.
Account Type(s):
Expense
Contract Start Date:
08/01/2015
Contract End Date:
07/31/2016
Current Contract Value:
$408,101
Expenditures:
$408,101

* Expenditures data includes accruals and are based on data through 30-Jun-2024.

BPA COR:
Env. Compliance Lead:
Work Order Task(s):
Contract Type:
Contract (IGC)
Pricing Method:
Cost Reimbursement (CNF)
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Viewing of Work Statement Elements

Deliverable Title WSE Sort Letter, Number, Title Start End Concluded
EC Documentation provided A: 165. Produce environmental compliance documentation for BPA 07/31/2016 07/31/2016
All administrative tasks fulfilled with timely quality products B: 119. Overall project administration 07/31/2016 07/31/2016
DNA from steelhead captured at weir collected C: 157. Collect adult DNA samples from steelhead 07/31/2016 07/31/2016
Spawning ground ecological data conducted D: 157. Conduct steelhead spawning ground surveys 05/31/2016 05/31/2016
DNA samples from age-1 steelhead parr collected E: 157. Collect DNA from age-1 steelhead parr 11/30/2015 11/30/2015
DNA samples from steelhead smolts collected F: 157. Collect DNA from steelhead smolts at smolt trap 07/01/2016 06/30/2016
Redd morphology and habitat data collected G: 157. Collect Ecological and Redd Morphology Data on the Spawning Grounds 05/31/2016 05/31/2016
Ecological traits influence of fitness H: 162. Analyze ecological data from spawning grounds 07/31/2016 07/31/2016
Produce accessible, error-checked datasets I: 157. Genotype Juvenile Steelhead and Conduct Parentage Analysis 07/31/2016 07/31/2016
Produce accessible, error-checked datasets J: 157. Genotype DNA Samples from Snake River Fall Chinook 07/31/2016 07/31/2016
Submit calendar year 2015 report to BPA L: 132. Submit Progress Report for Calendar Year 2015 05/01/2016 05/01/2016
Submit BiOp RPA Report in Taurus M: 202. BiOp RPA Report for CY 2015 03/15/2016 03/15/2016

Viewing of Implementation Metrics
Viewing of Environmental Metrics Customize

Primary Focal Species Work Statement Elements
Chinook (O. tshawytscha) - Snake River Fall ESU (Threatened)
  • 1 instance of WE 157 Collect/Generate/Validate Field and Lab Data
Steelhead (O. mykiss) - Upper Columbia River DPS (Threatened)
  • 6 instances of WE 157 Collect/Generate/Validate Field and Lab Data
  • 1 instance of WE 162 Analyze/Interpret Data

Sort WE ID WE Title NEPA NOAA USFWS NHPA Has Provisions Inadvertent Discovery Completed
A 165 Produce environmental compliance documentation for BPA 08/01/2015
B 119 Overall project administration 08/01/2015
C 157 Collect adult DNA samples from steelhead 04/25/2016
D 157 Conduct steelhead spawning ground surveys 04/25/2016
E 157 Collect DNA from age-1 steelhead parr 04/25/2016
F 157 Collect DNA from steelhead smolts at smolt trap 04/25/2016
G 157 Collect Ecological and Redd Morphology Data on the Spawning Grounds 04/25/2016
H 162 Analyze ecological data from spawning grounds 08/01/2015
I 157 Genotype Juvenile Steelhead and Conduct Parentage Analysis 04/25/2016
J 157 Genotype DNA Samples from Snake River Fall Chinook 04/25/2016
K 185 Periodic Status Reports for BPA 08/01/2015
L 132 Submit Progress Report for Calendar Year 2015 08/01/2015
M 202 BiOp RPA Report for CY 2015