The sponsors responded to the questions raised about this project in the preliminary review. The sponsor's response did not materially change the impression the ISRP has about this effort. The sponsor was asked to summarize the status of tissue collections and DNA genotyping of coho salmon. It was hoped that this would identify a deficiency in data from wild Columbia River coho, in contrast to other collections. The sponsor summarized limited published literature on genotyping coho salmon; it does not appear to be exhaustive, or recent. Most of the citations are from the early to mid-1990s. The sponsors were requested to provide evidence that Washington Department of Fish and Wildlife (WDFW) was a cooperator. They provided a letter indicating that WDFW would be willing to serve as the contractor of the genotyping if space and time is available in the laboratory. WDFW did not express particular interest in the work, and no information is provided on the genes that will be analyzed. Finally, the sponsors were asked to provide evidence of the need for the data. The response was not convincing. The coho status review that was cited was from 1995, not the more recent update, and there is no link to the Lower Columbia Salmon Recovery Plan, Lower Columbia Technical Recovery Team, or harvest management organization (Pacific Salmon Commission or Pacific Fishery Management Council) indicating that there is an urgent need for this data. In conclusion, the ISRP is unable to justify the need to collect this data, on the basis of the proposal.