The goal of this project is to asses nutrient availability within freshwater trophic levels of the Twisp River Watershed. Nutrient supplementation through specific prescriptions could follow if nutrients levels are shown to be limiting as a result of reduced, low levels of natural production within the basin.

This statement of work includes work elements for tasks to be completed in FY 2011. Sampling for primary, secondary and tertiary production will take place monthly, April - November. Sample sites will include another replicate transect 100 meters downstream where some of the sampling metrics will be measured to increase replication. BMI sampling will be increased to include a late fall and early spring event, this is based on trying to detect all cohorts in the BMI community.  Increased duration, decreased frequency and better spatial representation was based on ecological representation and statistical tests of 08, 09 and 2010  data, looking at variability and sample size efficiencies.

Fish sampling will be included in the SOW for this year, now that needed ESA permits are in place. Fish sampling for condition, growth and diet will be conducted at as many sites as possible. Tissue sampling is needed to conduct initial isotope baseline work.